Award details

Isolation and evaluation of simple sequence repeat-based markers for cereal genome research

Reference	PAG04430
Principal Investigator / Supervisor	Professor Robbie Waugh
Co-Investigators / Co-Supervisors	Dr Mike Gale
Institution	The James Hutton Institute
Department	Cell and Molecular Genetics Department
Funding type	Research
Value (£)	247,066
Status	Completed
Type	Research Grant
Start date	01/11/1995
End date	01/11/1998
Duration	36 months

Abstract

The potential of simple sequence repeat (SSR) based markers will be evaluated for applications in fundamental and applied aspects of cereal genome research. SSRs will be isolated in parallel from barley (SCRI) and wheat (JIC) and characterised. Pairs of oligonucleotide primers will be designed to the unique flanking sequences and used to amplify the corresponding locus from total DNA. The polymorphic information content of each marker (PIC-value) will be calculated using a set of genotypes chosen to best represent the genetic diversity present in the respective primary gene-pools. Transportability will be examined using accessions from the secondary and tertiary gene-pools and from related cereals and grasses. Polymorphic SSRs will be mapped intrachromasomally to existing segregating populations. The generated information will be assembled into a standard format database to facilitate general access.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Plant & Animal Genome Analysis (PAG) [1993-1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

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