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Gametophytic transposition as a tool for mutagenesis and gene mapping in plants
Reference
PAG01501
Principal Investigator / Supervisor
Professor John Draper
Co-Investigators /
Co-Supervisors
Professor Roderick Scott
Institution
Aberystwyth University
Department
Inst of Biological, Environ & Rural Sci
Funding type
Research
Value (£)
168,251
Status
Completed
Type
Research Grant
Start date
01/07/1994
End date
01/10/1998
Duration
51 months
Abstract
In a previous AFRC grant (PG91/505) we have shown that it is possible in tobacco to achieve high frequency, independent, germinal Ds transposition in the presence of Ac transposase gene expression from an early microspore- specific promoter. The current proposal aims to test the utility of this novel transposition system for the analysis of higher plant genomes in the future. Firstly, a gametophytic transposition system should greatly improve the efficiency of gene tagging programmes and thus a major objective of the research will be to test out this new technology in Arabidopsis and eventually to incorporate it into current gene tagging procedures. Secondly, we aim to demonstrate that it is possible to use high frequency transposition to linked genome sites to aid gene mapping, YAC library analysis and chromosome walking procedures in any transformable plant species.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Plant & Animal Genome Analysis (PAG) [1993-1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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