Award details

Plant golgi associated proteins - proteomics via viral expression

Reference	P20269
Principal Investigator / Supervisor	Professor Christopher Hawes
Co-Investigators / Co-Supervisors	Dr Petra Boevink, Professor Karl Oparka
Institution	Oxford Brookes University
Department	Faculty of Health and Life Sciences
Funding type	Research
Value (£)	193,075
Status	Completed
Type	Research Grant
Start date	01/03/2004
End date	30/06/2007
Duration	40 months

Abstract

This project will apply a new viral-based functional genomics approach, developed as SCRI, for the location of novel proteins associated with the plant Golgi apparatus, an organelle central to the processing of food materials in crop species. cDNA libraries from Arabidopsis tissues will be fused to the florescent protein and expressed en masse, using the viral-based vectors, in plants transformed with green fluorescent protein-tagged Golgi marker proteins. Lesions containing double-labelled Golgi will be excised, the cDNA sequenced and expressed via Agrobacterium infiltration in tobacco leaves. Proteins with confirmed association with the Golgi will be further characterised, to identify unique plant Gogli-specific and plant Gogli matrix proteins. Funds are also sought to establish a bioinformatics platform for dissemination of cDNA-GFP sequences.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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