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Discrimination between related anions nitrate and nitrite by bacterial environmental sensors and transport proteins
Reference
P20180
Principal Investigator / Supervisor
Professor Jeffrey Cole
Co-Investigators /
Co-Supervisors
Professor Steve Busby
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
209,006
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
This project will focus on two aspects of nitrate and nitrite utilisation: how nitrate and nitrite are transported across the E. coli cytoplasmic membrane; and how the presence of nitrate and nitrite ions is signalled in both E. coli and Neisseria gonorrhoeae. The first major objective is to identify the determinants of nitrate and nitrite transport in NarK, NarU and NirC from E. coli, and how the transporters distinguish between them. A bacterial two-hybrid system will be used to detect intra- and inter-subunit interactions within and between subunits of NarK, NarU and NirC. Further objectives will be to identify the determinants of the nitrate/nitrite specificity in NarX and NarQ; and reveal the molecular basis of the discrimination.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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