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Characterising G protein signalling components in Arabidopsis
Reference
P19863
Principal Investigator / Supervisor
Dr Richard Hooley
Co-Investigators /
Co-Supervisors
Institution
University of Bath
Department
Biology and Biochemistry
Funding type
Research
Value (£)
193,569
Status
Completed
Type
Research Grant
Start date
01/02/2004
End date
31/01/2007
Duration
36 months
Abstract
Plant heterotrimeric G proteins are involved in responses to light, pathogens and hormones, yet the molecular context in which they function is not understood. In this proposal, the yeast two-hybrid Ras recruitment system will be used to identify proteins that interact with GPA1. Interactions will be tested between GPA1, a chorismate mutase isoform, and a putative regulator of G protein signalling protein. Interactions will be confirmed in vitro and characterised in biochemical assays and by ectopic over-expression. Mutants will be isolated in proteins that interact with GPA1 and double mutants with other signalling mutants such as gpa1 generated. The phenotype and response of these to known G protein mediated events will be determined.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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