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Arabidopsis GCN2-related protein kinase a putative component of a general amino acid response signalling pathway
Reference
P19808
Principal Investigator / Supervisor
Professor Nigel Halford
Co-Investigators /
Co-Supervisors
Professor Martin Parry
,
Dr Matthew Paul
,
Dr Yuhua Zhang
Institution
Rothamsted Research
Department
Plant Biology & Crop Science
Funding type
Research
Value (£)
198,912
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
GCN2 is a yeast protein kinase that induces a general response to amino acid starvation involving changes in expression of many genes. We have cloned an Arabidopsis GCN2 homologue, AtGCN2, that complements the gcn2 mutation. Functional analyses of the AtGCN2 protein will be performed and the target site on its likely substrate, eIF-2alpha, will be identified. AtGCN2/eIF-2alpha interactions will be studied and other interacting proteins will be identified. Transgenic Arabidopsis plants with sense/RNAi AtGCN2 constructs will be produced and the expression of genes and levels of metabolites in these and controls in response to nitrogen supply and inhibitors will be determined by northern, RT-PCR and transcriptome analysis and metabolite profiling.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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