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Regulation of carbon partitioning from glucose-6-phosphate in heterotrophic plastids
Reference
P19448
Principal Investigator / Supervisor
Professor Alison Smith
Co-Investigators /
Co-Supervisors
Dr Stephen Rawsthorne
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
226,616
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
31/03/2007
Duration
42 months
Abstract
Heterotrophic plastids synthesise many important biochemicals (fatty acids, starch, amino acids, flavonoid precursors) using imported glucose-6-phosphate (Glc6P). We will investigate the regulation of partitioning of Glc6P using transgenic Brassica and Arabidopsis that have altered capacities for import of Glc6P into the plastid. We will measure the affect of altered Glc6P supply on storage product synthesis and on the metabolism of plastids isolated from rape embryos. Changes in metabolism in whole embryos will be measured through 13C-NMR spectroscopy and using metabolic profiling. Crosses between the transgenic Arabidopsis and other mutants in plastidic metabolism will be used to investigate interactions between these pathways in vivo.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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