Award details

Defining the sources of the acyl-CoA synthetase activity and their roles in acyl-CoA and fatty acid metabolism

Reference	P19408
Principal Investigator / Supervisor	Dr Mark Hooks
Co-Investigators / Co-Supervisors	Professor Ian Graham
Institution	Bangor University
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	263,464
Status	Completed
Type	Research Grant
Start date	01/08/2003
End date	30/11/2006
Duration	40 months

Abstract

A basic biochemical requirement for long-chain acyl-CoA synthetase (LACS) activity is the activation fatty acids for lipid synthesis and fatty acid degradation. Virtually nothing is known about the existence or function of medium- (MACS) and short- chain (SACS) activities. Our finding is that an AMP binding protein (AMPBP) has SACS activity prompts us to hypothesise that other AMPBPs may encode ACSs. The AMPBPs will be expressed in vitro and the substrate specificities determined. We will determine the tissue expression patterns and subcellular location to define functions. We will profile acyl-CoAs and fatty acids in tissues of plants lacking each enzyme activity. The results will provide insight regarding the metabolism of acyl- CoAs, and the mechanisms controlling acyl-CoA and fatty acid levels. The source materials will be full-length AMPBP cDNAs and their corresponding knock-outs.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search