Award details

The role of the chloroplast protein CP12 in the regulation of plant growth and metabolism

Reference	P19404
Principal Investigator / Supervisor	Professor William Quick
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Animal and Plant Sciences
Funding type	Research
Value (£)	59,540
Status	Completed
Type	Research Grant
Start date	02/02/2004
End date	01/05/2007
Duration	39 months

Abstract

CP12 has been shown to form a regulatory complex with the Calvin cycle enzymes, glyceraldehyde-3-phosphate dehydrogenase and ribulose-5-phosphate kinase in vitro. Antisense transgenic tobacco plants will be used to investigate the in vivo role of CP12 in the regulation of photosynthetic carbon assimilation and on partitioning of carbon to sucrose, starch and secondary products. The effect of reductions in CP12 protein on complex formation and NADP-GAPDH and PRKase activity and regulation will be quantified during light/dark transitions. To address the impact of perturbations in the regulation of these Calvin cycle enzymes on carbon assimilation, the response of photosynthesis to changes in light intensity and CO2 concentration and during light induction will be measured. Photosynthetic and non-photosynthetic carbon fluxes will be determined using 14C feeding. Changes in primary and secondary metabolic products will be measured using freeze clamp techniques and LC-Tof-MS analysis. (Joint with grant number 19403).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

P19403 The role of the chloroplast proteinCP12 in the regulation of carbon metabolism

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