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In vivo mapping of N-metabolism during foraging for patchy resources by cord-forming basidiomy- cetes

Reference	P19284
Principal Investigator / Supervisor	Professor Mark Fricker
Co-Investigators / Co-Supervisors	Dr Peter Darrah, Professor Richard Ratcliffe, Dr Monika Tlalka, Dr Sarah Caroline Watkinson
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	216,052
Status	Completed
Type	Research Grant
Start date	22/03/2003
End date	21/08/2007
Duration	53 months

Abstract

Saprophytic cord-forming basidiomycetes play a central role in nutrient cycling in terrestrial ecosystems. We will define the complex patterns of N-redistribution in Phanerochaete velutina and Serpula lacrymans developing in a patchy resource environment using photon-counting scintillation imaging. The spatial pattern of expression of key genes involved in N- metabolism, particularly on the urea cycle, and N-regulation (areA) will be mapped using RT-PCR. Metabolite levels will be quantified using NMR in parallel. The role of the dynamic vacuolar network in long-distance N-transport will be determined using 4-D confocal microscopy and fluorescence recovery after photobleaching. Results will be integrated using an in silico simulation approach.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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