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Enzyme-catalysed N-acyl homoserine lactone degradation in Erwinia carotovora subsp. atroseptica

Reference	P19162
Principal Investigator / Supervisor	Dr Martin Welch
Co-Investigators / Co-Supervisors	Professor David Spring
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	193,192
Status	Completed
Type	Research Grant
Start date	13/10/2003
End date	12/05/2007
Duration	43 months

Abstract

A. tumefaciens expresses an enzyme (AttM) that is capable of degrading the endogenous quorum-sensing signalling molecule. The recently completed genome sequence of Erwinia carotovora subsp. atroseptica (Eca) reveals four orthologs of attM and several candidate regulators of these genes. We will (1) systematically inactive these orthologs (and their potential regulators) by marker exchange, (2) examine the effects of these mutations on quorum- sensing-dependent phenotypes, (3) correlate changes in the proteome/metabolome of the mutants with the in vitro biochemical activity of the enzymes encoded by the orthologs, (4) use mutagenesis to screen Eca for novel mechanisms of N-AHL degradation.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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