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Identification of Arabidopsis proteins targeted by a jasmonate signal to the SCF-COI1 ubiquitin ligase

Reference	P18585
Principal Investigator / Supervisor	Professor John Turner
Co-Investigators / Co-Supervisors	Professor Alessandra Devoto
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	280,280
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2006
Duration	36 months

Abstract

F-box proteins recruit substrate proteins for ubiquitin (Ub)-mediated proteolysis. COI1 is an F-box protein required for jasmonate (JA)- regulated defence and fertility, and we predicted that it recruits regulators of JA responses to Ub-mediated proteolysis. Here we propose to identify proteins that are ubiquitinated by the COI1 Ub- ligase. Proteins that immunoprecipitate with epitope-tagged COI1, and proteins that immunoprecipitate with epitope-tagged Ub in wild plants but not coi1 mutants, will be purified and identified by mas spectrometry. From these candidates, substrates for COI1-dependent proteolysis will be identified by their more rapid disappearance, as GUS fusions, from wild-type plants than from coi1 mutants.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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