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The function of LuxS and its cognate autoinducer in bacterial central metabolism
Reference
P18575
Principal Investigator / Supervisor
Professor Kim Hardie
Co-Investigators /
Co-Supervisors
Professor David Barrett
,
Professor Paul Williams
,
Dr Klaus Winzer
Institution
University of Nottingham
Department
Sch of Pharmacy
Funding type
Research
Value (£)
73,060
Status
Completed
Type
Research Grant
Start date
15/09/2003
End date
14/03/2005
Duration
18 months
Abstract
Formation of the bacterial signal molecule AI-2 is catalysed by the LuxS protein which also fulfils a metabolic function in the activated methyl cycle. The respective contributions of disruption of quorum sensing and the activated methyl cycle to the phenotype of an E. coli Lux1 mutant will be delineated by metabolic profiling using LC and GC-MS based technology together with metabolic flux ratio analysis. Correlation of metabolite pools, metabolic flux through the cycle, and AI-2 production with growth conditions and the physiological state of the cell will reveal the kind of information exchanged by AI-2 signalling. Metabolic changes observed in luxS mutants will contribute to the identification of phenotypes not linked to quorum sensing.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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