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Development of a regulated expression system for use in mycobacteria
Reference
P18543
Principal Investigator / Supervisor
Dr Brian Robertson
Co-Investigators /
Co-Supervisors
Dr Helen Murphy
,
Professor Douglas Young
Institution
Imperial College London
Department
Dept of Medicine
Funding type
Research
Value (£)
129,940
Status
Completed
Type
Research Grant
Start date
17/02/2003
End date
17/02/2005
Duration
24 months
Abstract
We propose to build on preliminary evidence that tetracycline- dependent regulator from Corynebacterium glutamicum can function as an inducible expression system in mycobacteria. We will explore the hypothesis that the regulatory mechanism involves Tet-induced activation rather than the more common release of repression, and will use this information to optimise the expression system. We will test the use of the Tet system by analysing regulated expression of a mycobacterial p97/NSF homologue and an essential superoxide dismutase enzyme in vitro cultures of slow-growing and rapid- growing mycobacteria, and by measuring luminescent activity of reporter constructs in a mouse infection model. We anticipate that the Tet system will provide important new tool for mycobacterial functional genomics.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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