Award details

Mechanism of a novel nitric oxide sensor in Escherichia coli

Reference	P18536
Principal Investigator / Supervisor	Dr S Spiro
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	197,088
Status	Completed
Type	Research Grant
Start date	12/05/2003
End date	11/01/2004
Duration	8 months

Abstract

The NorR regulatory protein of Escherichia coli activates expression of a flavorubredoxin (encoded by the norV gene) that is required for resistance to nitric oxide (NO) and reactive nitrogen intermediates (RNIs). NoR activity is only apparent in cells exposed to sources of NO and RNIs, thus NorR functions as a biological sensor of reactive N compounds. Genetic techniques will be used to establish whether there are any other E. coli genes that are required for NorR activity. The NoR protein will be purified and DNA binding and in vitro transcription assays used to identify the signal that is required to induce NorR activity. The signalling domain of NorR will be expressed and purified in isolation for biochemical analysis. A NorR-dependent promoter (from the norV gene) will be characterised, and other targets (if any) for NorR activation will be identified.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/A51065X/1 Mechanism of a nitric oxide sensor in Escherichia coli

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