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Functional analysis of TIO an essential gene required for gametophytic cytokinesis

Reference	P18532
Principal Investigator / Supervisor	Professor David Twell
Co-Investigators / Co-Supervisors	Dr Sung-Aeong Oh
Institution	University of Leicester
Department	Biology
Funding type	Research
Value (£)	248,004
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2006
Duration	36 months

Abstract

Asymmetric division is an important mechanism in generating cellular pattern and plant architecture. Pollen mitosis I is an intrinsically asymmetric division that is essential for correct specification of pollen cell fate and differentiation of the male gametes. We have identified three alleli, two-in-one (tio) mutations that cause specific defects in gametophytic cytokinesis. Positional cloning has revealed that TIO encodes a unique protein kinase in plants. We propose to investigate TIO functions to improve our understanding of the mechanisms which operate to control asymmetric division and gametophytic cytokinesis in planta. We propose: (i) functional domain analysis of TIO; (ii) detailed cytological analysis of cytokinesis in gametophytic and sporophytic cell types using GFP-marker technology; (iii) identification of TIO- interacting proteins.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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