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Gene silencing in the true obligate barley powdery mildew fungus
Reference
P18308
Principal Investigator / Supervisor
Professor Sarah Gurr
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
142,044
Status
Completed
Type
Research Grant
Start date
01/05/2003
End date
31/12/2005
Duration
32 months
Abstract
Our search for RNAi in Blumeria graminis hordei reveals evidence by inference, notably homologues of the Neurospora crassa quelling genes, qde 2 and qde 3. Here, we seek evidence by experiment by exploiting methanol inducible expression and subsequent silencing of GFP (which we have demonstrated in Pichia) and thence the silencing of the Bgh catB, cPKA and cutA genes. Each proposed gene to be silenced meets the qualifying criteria, notably, is single copy, shows evidence for involvement in differentiation and/or pathogenicity, carries a known RT-PCR transcript profile (deemed important for activation of the switch at specific time-points), and its protein product is detectable.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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