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The identification of transcription factors regulating the expression of NRT2 nitrate transporter genes in Arabidopsis
Reference
P18298
Principal Investigator / Supervisor
Professor Brian Gordon Forde
Co-Investigators /
Co-Supervisors
Institution
Lancaster University
Department
Lancaster Environment Centre
Funding type
Research
Value (£)
58,224
Status
Completed
Type
Research Grant
Start date
14/10/2003
End date
13/10/2004
Duration
12 months
Abstract
Members of the NRT2 family of nitrate transporter genes are highly regulated. We will use the Arabidopsis AtNRT2.1 gene in a pilot project to examine the potential of surface plasmon resonance (SPR) as a proteomics tool for the identification of transcription factors interacting with defined sequences within plant promoters. DNA fragments and oligonucleotides from the upstream region of AtNRT2.1 will be immobilised on biosensors and protein mixtures from nuclear extracts will be tested for their ability to interact with these in a sequence-specific manner. The association and dissociation kinetics will be established and DNA-binding proteins of interest will be purified using the biosensor as an affinity matrix and identified by MALDI-TOF.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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