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Discovery of genes controlling solute accumulation by random gene activation specifically in the stele of Arabidopsis
Reference
P18220
Principal Investigator / Supervisor
Professor Roger Leigh
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
177,668
Status
Completed
Type
Research Grant
Start date
15/02/2003
End date
14/02/2006
Duration
36 months
Abstract
Control of xylem Na+ by cells adjacent to the xylem is proposed to be important in determining shoot Na+ accumulation and thus plant salinity tolerance. To test this hypothesis, expression of random genes will be altered specifically in the stele of Arabidopsis by transforming a line of Arabidopsis expressing the yeast transcription factor, GAL4, specifically in the stele with the GAL4 upstream activating sequence. This will activate transcription of genes near the site of the insertion of this construct. Activated genes will be identified using TAIL-PCR in tolerant lines that also show reduced levels of accumulation of Na+. This strategy provides a novel approach for discovering key genes involved in processes adjacent to the xylem.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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