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Identifying novel genes required for gene silencing in Arabidopsis
Reference
P17832
Principal Investigator / Supervisor
Professor Tamas Dalmay
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
158,752
Status
Completed
Type
Research Grant
Start date
03/03/2003
End date
02/06/2006
Duration
39 months
Abstract
Gene silencing is a response to various types of foreign nucleic acid including viruses, transposons, transgenes and double stranded (ds) RNA. Several mutants, disabled in gene silencing, have been identified in two independent genetic screens using the model plant Arabidopsis. However, silencing can be induced on all of these mutant plants by a virus or by hairpin structure transgenes (producing high amount of dsRNA). These and other data suggest that all the identified genes are involved only in the amplification and maintenance step. The aim of this project is to find genes required for other steps in the process, for example: degradation of dsRNA or ssRNA. Seeds will be mutagenised and M2 plants will be screened for disability in silencing by a hairpin structure transgene. The mutant genes will be identified by map-based cloning.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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