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Tobacco calcium dependent protein kinase-7:a critical role in male generative cell cytokinesis?
Reference
P17800
Principal Investigator / Supervisor
Professor Tony Trewavas
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Cell and Molecular Biology
Funding type
Research
Value (£)
174,808
Status
Completed
Type
Research Grant
Start date
01/08/2003
End date
30/09/2004
Duration
14 months
Abstract
Transfection of pollen tubes with aequorin has detected a transient elevation of cytosol calcium during generative cell binary. Tobacco CDPK-7 expression is limited to late anthesis fission and pollen tube growth and fusion constructs indicate the protein kinase is specifically targeted to the male generative cell membrane. Using state of the art proteomics, the protein substrates and their amino acid phosphorylation sites for CDPK-7 in the generative cell membrane will be identified and phosphorylation status estimated during cytokinesis. Mis expression of CDPK-7 and photolysis of loaded caged calcium will be used to investigate the regulation of pollen tube cytokinesis by the cytosol calcium transduction pathway.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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