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The plant 'nutriome' accessed by an ICP-MS screen for unusual element accumulation in activation tagged Arabidopsis mutants

Reference	P17194
Principal Investigator / Supervisor	Professor Roger Leigh
Co-Investigators / Co-Supervisors	Professor Sally Gibson, Professor Stephen Paul McGrath, Professor Mark Tester
Institution	University of Cambridge
Department	Plant Sciences
Funding type	Research
Value (£)	387,316
Status	Completed
Type	Research Grant
Start date	01/02/2003
End date	30/04/2006
Duration	39 months

Abstract

The aim is to provide a resource for the identification of genes controlling the accumulation of nutrients and inorganic toxins (the nutriome) in higher plants by screening activation tagged lines of Arabidopsis for unusual concentrations of nutrients. Up to 50,000 lines will be grown in soil to which sub-toxic concentrations of a range of inorganic ions will be added. Shoot tissue of 3-week old plants will be harvested, digested and assayed simultaneously for 24 elements using an inductively-coupled plasma spectrometer - mass spectrometer (ICP- MS). The data will be analysed using bioinformatic approaches and made freely available to the scientific community via a website as well as in collaboration with a complementary project in the US. Identified mutants will subsequently be used in our laboratories for the study of mechanisms controlling accumulation of K+,Na+,Cl, and B(OH)3 while UK groups who have supported this application will use them in studies of a number of other essential and/or potentially toxic ions and trace metals.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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