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Spatial and temporal expression of rhi genes: determining the contribution of rhi to rhizosphere fitness

Reference	P16688
Principal Investigator / Supervisor	Professor Chris Leaver
Co-Investigators / Co-Supervisors	Dr Andrew Spiers
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	212,040
Status	Completed
Type	Research Grant
Start date	18/06/2002
End date	17/06/2005
Duration	36 months

Abstract

Application of IVET to study rhizosphere colonisation by Pseudomonas fluorescens has revealed more than 80 rhizosphere-inducible (rhi) genes. Understanding their contribution to ecological performance is essential. Typically this is determined by competition experiments between wild type and rhi mutants, but this approach is flawed because it fails to take into consideration the complexity of the rhizosphere. Using the RIVET strategy we propose to determine the spatial and temporal distribution of rhi-inducing conditions in situ. This will generate hypotheses concerning the activity of rhi genes that will be tested using constitutive-rfp, inducible-gfp, reporters. The contribution of rhi genes to fitness will then be determined at the microscopic level in precisely that niche where inducting signals are known to be present.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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