BBSRC Portfolio Analyser
Award details
Coupled translational termination and re- initiation of overlapping open reading frames
Reference
P16683
Principal Investigator / Supervisor
Professor Andrew Easton
Co-Investigators /
Co-Supervisors
Institution
University of Warwick
Department
Biological Sciences
Funding type
Research
Value (£)
163,024
Status
Completed
Type
Research Grant
Start date
01/11/2002
End date
01/11/2005
Duration
36 months
Abstract
The M2 gene of human respiratory syncytial virus contains two ORFs which are translationally coupled. The ribosome departing from ORF-1 moves in the 5' direction and re-initiates translation of ORF-2. The mechanism of this process will be investigated for related pneumoviruses. The sequence and distance constraints of the overlap region between the ORFs will be investigated with particular attention paid to regions of predicted secondary structure. In vitro transcription analyses will be used to characterise the coupling process and the positions of apused ribosomes will be determined and quantitated using a toeprinting method. The potential for the coupling process to permit the regulated expression of two gene products will be assessed.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search