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Use of ABC transporter binding proteins of S. meliloti as biosensors of the rhizosphere environment
Reference
P16655
Principal Investigator / Supervisor
Professor Philip Poole
Co-Investigators /
Co-Supervisors
Institution
University of Reading
Department
Animal and Microbial Sciences
Funding type
Research
Value (£)
242,392
Status
Completed
Type
Research Grant
Start date
01/05/2002
End date
30/04/2005
Duration
36 months
Abstract
The rhizosphere is one of the most complex yet important of environments for both microbial and plant growth. One of the most powerful tools that could be developed to study this environment is a set of molecular biosensors to monitor environmental conditions temporally and spatially. Total genome analysis of the S. meliloti and M. loti genomes reveals an explosive growth in the number of ABC uptake systems in these organisms. The binding proteins of these transporters are highly solute specific and tightly induced in response to appropriate conditions. We propose to develop a complete set of ABC-binding protein-Gfp fusions in the pOT vectors developed in our lab to globally examine the conditions required for induction of these fusions and how their expression varies temporally and spatially in the rhizosphere.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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