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Proteomic analysis of cyclin-dependent kinase complexes in synchronized Arabidopsis cells

Reference	P16578
Principal Investigator / Supervisor	Professor James Murray
Co-Investigators / Co-Supervisors	Professor Paul Dupree
Institution	University of Cambridge
Department	Institute of Biotechnology
Funding type	Research
Value (£)	175,156
Status	Completed
Type	Research Grant
Start date	01/01/2002
End date	01/01/2004
Duration	24 months

Abstract

Cell division plays key roles in plant growth and development, and cell cycle controls respond to extrinsic and intrinsic signals, including hormones. Cell cycle regulation is mediated by cyclin-dependent kinase (CDK) complexes, whose abundance, composition, and post-translational state alters through the cell cycle. Here we propose the first global analysis of CDK complexes in any plant by 2D-electrophoresis and mass spectrometry, making use of unique cell synchrony systems developed for Arabidopsis cells by the applicant. The effects on CDK complexes of exogenous application of hormones or of altered hormone balance in transgenic or mutant plants will link our knowledge of the effects of hormones and cell division, revealing hormone targets in cell cycle control and integrating the biochemistry of CDKs with whole plant responses.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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