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Desulphonation of arylsulphonate xenobiotics
Reference
P16521
Principal Investigator / Supervisor
Dr Michael Kertesz
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
213,868
Status
Completed
Type
Research Grant
Start date
18/02/2002
End date
18/02/2005
Duration
36 months
Abstract
Aromatic sulphonates are commercially important xenobiotics used as surfactants, dyestuffs and cement additives. As part of their microbial biodegradation they are desulphurised by an FMNH2-dependent mono-oxygenase, which forms part of a large sulphonatase enzyme complex. This complex will be characterised by cross-linking and mass spec. to determine the important protein interactions for desulphonation. The enzyme components of the complex will be purified, and their redox and catalytic properties characterised. Structural characterisation will be carried out by X-ray crystallography of the individual components. Regulation of sulphonatase gene expression by the CysB and AsfR regulator proteins will be studied at a molecular level, yielding important new data on sulphur starvation physiology in environmental bacteria.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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