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The physiological roles of gibberellin 20-oxidase isozymes in plant development

Reference	P16508
Principal Investigator / Supervisor	Professor Peter Hedden
Co-Investigators / Co-Supervisors	Dr Konstantin Kanyuka, Dr Andrew Phillips
Institution	Rothamsted Research
Department	Plant Biology & Crop Science
Funding type	Research
Value (£)	165,672
Status	Completed
Type	Research Grant
Start date	22/04/2002
End date	21/07/2005
Duration	39 months

Abstract

The biosynthesis of the gibberellin (GA) hormones is regulated by intrinsic and environmental factors, many of which act on GA 20-oxidase (GA20ox), a rate-limiting enzyme that functions late in the pathway. The Arabidopsis genome contains five putative GA20ox genes, some of which have been shown to be expressed in a distinct tissue-specific manner. We will test the hypothesis that these genes are involved in different developmental processes, such as stem extension, flower development and seed germination, by isolating lines with knock-out mutations in each GA20ox gene, either by screening populations of T-DNA insertion lines, or by specific gene silencing using RNA interference. We will correlate the phenotypes of the mutants with their gene expression patterns, determined by in situ hybridisation and immunolocalisation.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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