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Molecular basis of changes in resource allocation induced by antisense SnRK1 gene expression and environmental stress
Reference
P16424
Principal Investigator / Supervisor
Professor Nigel Halford
Co-Investigators /
Co-Supervisors
Dr Matthew Paul
Institution
Rothamsted Research
Department
Plant Biology & Crop Science
Funding type
Research
Value (£)
130,608
Status
Completed
Type
Research Grant
Start date
01/03/2002
End date
28/02/2006
Duration
48 months
Abstract
Antisense SnRK1 gene expression in potato leaves affects partitioning between leaves (sources) and tubers (sinks) under low light. We propose two hypotheses: 1. This effect arises through deregulation of sucrose phosphate synthase (SPS), sucrose synthase (SuSy) and other enzymes of carbon metabolism; 2. SnRK1 may be involved in adaptations to other environmental changes (release from nutrient or temperature stress) that require remobilization of carbon. These hypotheses will be tested by growing wild type and transgenic plants under different light conditions, and by switching plants between nutrient deprivation and availability and from low to optimum temperature. SnRK1, SPS, SuSy, nitrate reductase, alpha amylase and other enzymes of carbon metabolism will be analysed at the level of gene expression, activation state and activity.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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