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Developing Pichia pastoris as a heterologous expression system for ion transporters
Reference
P16351
Principal Investigator / Supervisor
Dr Stephen Roberts
Co-Investigators /
Co-Supervisors
Professor Brian Gordon Forde
Institution
Lancaster University
Department
Biological Sciences
Funding type
Research
Value (£)
105,848
Status
Completed
Type
Research Grant
Start date
01/01/2003
End date
31/03/2005
Duration
27 months
Abstract
Despite the existence of a number of heterologous expression systems for the characterisation of ion transporters, low expression levels can preclude the electrophysiological analysis of some ion transporters; consequently, there is a growing requirement for the development of alternative expression systems. High expression levels can be achieved using the methylotropic yeast, Pichia pastoris and the aim of the proposal is to 1) establish the patch clamp technique for its use on Pichia and 2) express HAK1 and LCT1 (plant transporters requiring high expression levels) in Pichia to enable their electrophysiological characterisation with respect to their modes of ion coupling, response to voltage and regulation by cytosolic factors.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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