Award details

Alternative pathway of protein import into plastids

Reference	P15823
Principal Investigator / Supervisor	Dr Caroline Bowsher
Co-Investigators / Co-Supervisors	Professor Stephen High
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	210,092
Status	Completed
Type	Research Grant
Start date	01/09/2002
End date	28/02/2006
Duration	42 months

Abstract

There is evidence that there are significant differences between the import of proteins into root plastids and chloroplasts, and that there is more than one import pathway. This project aims to elucidate the biochemical basis for different protein import pathways and to identify and characterise novel components of the protein translocation machinery from root plastids. A number of available precursors will be used to study the kinetics of protein import. Novel components of the root plastid translocation apparatus will be identified by cross- linking of radiolabelled precursors and label-transfer studies, coupled with sensitive mass spectrometry (MALDI/Q-TOF). Transit peptide domains important in targeting to this new pathway will be identified by deletion analysis and domain swapping of root and leaf ferredoxin precursor sequences.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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