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Metal recognition and transcriptional activation by the CueR regulator

ReferenceP15753
Principal Investigator / Supervisor Dr Jonathan Hobman
Co-Investigators /
Co-Supervisors
Institution University of Birmingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 204,824
StatusCompleted
TypeResearch Grant
Start date 01/04/2002
End date 01/04/2005
Duration36 months

Abstract

The CueR regulator of Escherichia coli is a member of the MerR family of transcriptional regulators; it responds to copper and silver salts and controls expression of the major copper export gene (copA) among other genes. The recently-solved structure of one conformation of one member of the MerR family; BmrR, a dye-responsive regulator from Bacillus, has given some further insight into how this family of regulators may cause transcriptional activation. We propose to make mutants of CueR to elucidate the mechanisms of discriminatory recognition of copper and other metals, and to determine the mechanism of gene regulation by this new and experimentally tractable regulator. This will test the current models for the mechanisms of metal-responsive transcriptional activation by MerR family regulators.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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