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Assessing the potential for increasing photosynthetic carbon fixation in transgenic tobacco plants over-expressing SBPase

Reference	P15748
Principal Investigator / Supervisor	Professor Christine Raines
Co-Investigators / Co-Supervisors	Professor Richard Leegood, Dr Julie Lloyd, Professor William Quick
Institution	University of Essex
Department	Biological Sciences
Funding type	Research
Value (£)	164,617
Status	Completed
Type	Research Grant
Start date	07/01/2002
End date	06/06/2005
Duration	41 months

Abstract

Transgenic tobacco plants over-expressing wild type or a fully active, deregulated, SBPase will be studied to assess the impact of altered SBPase activity on the flux of carbon through the Calvin cycle and on partitioning of carbon to starch and sucrose. Photosynthetic carbon fixation will be measured in SBPase transgenic plants grown in a range of light and temperature regimes and under elevated CO2. Enzyme activities, metabolite levels and non structural carbohydrate levels will be determined. 14CO2 feeding experiments will be carried out to provide information on carbon fluxes through the cycle and on export of carbon for sucrose, starch and shikimic acid biosynthesis. This will provide information to allow a strategy to increase photosynthetic capacity by manipulating primary carbon fixation to be developed.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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