Award details

DNA packaging in Streptomyces phage; tools for rapid gene transfer directed evolution and genetic prospecting

Reference	P15574
Principal Investigator / Supervisor	Professor Margaret Smith
Co-Investigators / Co-Supervisors	Mr Christopher Finnis
Institution	University of Nottingham
Department	Sch of Biology
Funding type	Research
Value (£)	218,632
Status	Completed
Type	Research Grant
Start date	01/03/2002
End date	31/08/2003
Duration	18 months

Abstract

Actinomycetes, especially Streptomyces species, are major producers of commercially important secondary metabolites. A strain of S. coelicolor has been developed for in vivo cosmid packaging into phiC31 phage particles. This project aims to optimise cosmid packaging in vivo and to develop conditions for in vitro packaging. In addition, the host range of cosmid delivery will be investigated and the feasibility of expression in antibiotic biosynthesis genes or global regulators in many different species will be demonstrated. These new genetic tools should facilitate screening of combinatorial, directed evolution or metagenome libraries in many different genetic backgrounds for synthesis of novel secondary metabolites.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BBS/X/00069 DNA packaging in Streptomyces phage tools for rapid gene transfer directed evolution and genetic prospecting

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