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Biosynthesis of mupirocin by Pseudomonas fluorescens

Reference	P15257
Principal Investigator / Supervisor	Professor Christopher Thomas
Co-Investigators / Co-Supervisors	Dr Joanne Hothersall, Professor Thomas Simpson
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	269,452
Status	Completed
Type	Research Grant
Start date	01/06/2001
End date	01/06/2004
Duration	36 months

Abstract

Having the complete sequence of the mupirocin biosynthetic genes from Pseudomonas fluorescens as a result of current BBSRC support the new project will establish the relationship between genes of the cluster and enzymic steps in the biosynthetic pathway. This will involve subcloning segments of the cluster as well as site-directed mutations, followed by HPLC analysis of novel intermediates. The second stage will be to determine whether we can create novel biologically active compounds by manipulating the cluster, either through alteration of individual enzyme centres or by replacement of subsegments of the region by subpathways from other sources and which could be joined to either the monic acid or the 9-hydroxy- nonanoic acid moiety. Finally we will overproduce and set up crystallisation trials on key proteins in the pathway with a view to structural studies.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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