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Functional dissection of bacterial and chloroplast protein translocation mechanisms using GFP imaging
Reference
P15253
Principal Investigator / Supervisor
Professor Colin Robinson
Co-Investigators /
Co-Supervisors
Institution
University of Warwick
Department
Biological Sciences
Funding type
Research
Value (£)
193,564
Status
Completed
Type
Research Grant
Start date
01/11/2001
End date
31/10/2005
Duration
48 months
Abstract
We have determined the subunit composition of the twin-arginine translocation (Tat) protein transporter from Escherichia coli, and have developed a highly effective GFP imaging system to assay its activity in vivo. This proposal aims to determine the roles of the individual Tat subunits through a systematic mutagenesis of the tatABC operon. Using imaging, we will: (i) generate and analyse mutants that are defective in Tat-dependent export and (ii), identify mutations that specifically block substrate binding and (iii) substrate translocation by the Tat system; and (iv) we will visualise the in vitro imports of GFP into chloroplast thylakoids.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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