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Investigation of the p52 MAPK signalling cascade involved in the SI response in Papaver rhoeas

Reference	P15216
Principal Investigator / Supervisor	Professor Vernonica E. (Noni) Franklin-Tong
Co-Investigators / Co-Supervisors	Professor Chris Franklin
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	105,460
Status	Completed
Type	Research Grant
Start date	01/10/2001
End date	01/12/2003
Duration	26 months

Abstract

We have recently identified a 52 kDa pollen protein with MAPK activity that is activated in the SI response. In this proposal we aim to (i) Clone and characterise the SI-induced p52-MAPK. (ii) Analyse the signalling pathways stimulating p52 MAPK activation. (iii) Identify proteins that interact with the p52-MAPK using 2- hybrid cloning and pull-down strategies. (iv) To begin the analysis of the interacting proteins using proteomic approaches. Overall these studies will provide significant insights into how the components of the SI signalling pathway are integrated to mediate the specific inhibition of incompatible pollen. Moreover they are likely to provide further insights into general stress responses and signalling cascades.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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