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Generating synthetic disease resistance genes by PCR-mediated gene shuffling of tomato Cf homologues
Reference
P15199
Principal Investigator / Supervisor
Dr Colwyn Thomas
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Biological Sciences
Funding type
Research
Value (£)
185,468
Status
Completed
Type
Research Grant
Start date
30/04/2001
End date
29/12/2004
Duration
44 months
Abstract
PCR-mediated gene shuffling is a powerful technique for generating chimeras that combine useful mutations from individual genes. The tomato Cf-4 and Cf-9 genes and their homologues (Hcr9s) will be shuffled using an error-prone DNA polymerase. This process will mimic in vitro the natural mechanisms that generate novel recognition specificities in this R gene class but at a vastly accelerated rate. Libraries of recombinant Hcr9s will be tested in high throughput transient expression assays for their ability to recognise defined C. fulvum extracellular proteins (ECPs) and to induce a hypersensitive response. This will determine the feasibility of creating synthetic plant resistance genes and is important to the goal of engineering durable resistance in crop plants.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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