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Molecular analysis of the pathway of bacterial thiosulphate and sulphide oxidation

ReferenceP15195
Principal Investigator / Supervisor Professor Benjamin Berks
Co-Investigators /
Co-Supervisors
Institution University of Oxford
DepartmentBiochemistry
Funding typeResearch
Value (£) 216,012
StatusCompleted
TypeResearch Grant
Start date 30/09/2002
End date 29/01/2006
Duration40 months

Abstract

The Sox system is used by both photosynthetic and non-photosynthetic bacteria to oxidize thiosulphate and sulphide to sulphate. Building upon our BBSRC-funded studies with the model organism Rhodovulum sulfidophilum we aim to elucidate the reactions of the Sox pathway using a combination of biochemical and genetic techniques. The transformations of protein-bound sulphur species will be tracked using mass spectrometry and radiolabelling approaches. The connections between the mechanisms of thiosulphate and sulphide oxidation will be probed including a genetic analysis of the proposal that flavocytochrome c is a sulphide dehydrogenase. The role of a disulphide bond reducing system in the Sox pathway will be investigated.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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