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Molecular analysis and exploitation of Ry-mediated resistance

Reference	P15172
Principal Investigator / Supervisor	Professor Sir David Baulcombe
Co-Investigators / Co-Supervisors	Dr Pere Mestre
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	200,996
Status	Completed
Type	Research Grant
Start date	26/04/2001
End date	26/04/2004
Duration	36 months

Abstract

Using genetic mapping and transient assay procedures the Ry PVY resistance gene has been localised to a 25kb region in the potato genome that does not contain typical NBS/LRR resistance genes. We shall investigate each of the four genes in the 25kb Ry region to find out whether they act either individually or in combination to mediate resistance. We shall characterise the gene(s) with the Ry function and investigate the nature of the interaction with the viral elicitor of resistance using yeast two hybrid and immuno- precipitation based methods. To assess the similarity of Ry and other resistance mechanisms we shall investigate and compare the components of the signalling pathways using chemical inhibitors and virus- induced silencing of candidate genes. Finally we shall investigate the functionality of Ry as a transgene in different potato cultivars and other solanaceae.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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