BBSRC Portfolio Analyser
Award details
Type III secretion of effector proteins by Pseudomonas syringae
Reference
P15063
Principal Investigator / Supervisor
Professor John Mansfield
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
180,124
Status
Completed
Type
Research Grant
Start date
01/04/2001
End date
01/05/2004
Duration
37 months
Abstract
The structure and function of the type III secretion system which delivers effector proteins from bacteria into plant cells will be examined. Expression by Pseudomonas syringae pv. phaseolicola of hrp genes encoding the type III architecture and avr and vir genes (encoding effectors) will be examined in vitro and in the plant using real time RT-PCR and a boutique DNA microarray. Secretion complexes will be isolated for immunocytochemistry and the movement of proteins through the Hrp pilus examined using epitope-tagged HrpA, HrpZ and AvrPphE. Protein and mRNA-located signals for secretion into and through the type III system will be analysed by domain swapping experiments and by use of mutagenized forms of the effector AvrPphE.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search