Award details

RNA polymerase and transcription factors in hammerhead viroid replication in chloroplasts

Reference	P15034
Principal Investigator / Supervisor	Professor Kenneth Buck
Co-Investigators / Co-Supervisors	Professor Martin Buck, Dr Robert Coutts
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	182,840
Status	Completed
Type	Research Grant
Start date	01/12/2001
End date	01/12/2004
Duration	36 months

Abstract

Hammerhead viroids replicate in chloroplasts, but it is not known which chloroplast RNA polymerase is responsible for their replication: the multisubunit plastid-encoded RNA polymerase (PEP), which is similar to bacterial RNA polymerases, the single-subunit nuclear-encoded RNA polymerase (NEP, similar to phage T7 RNA polymerase) or a third polymerase. Viroid replication complexes and RNA polymerases from uninfected chloroplasts will be isolated and purified. Proteins in the purified replication complex will be compared with chloroplast RNA polymerase subunits and transcription factors. An in vitro replication system will be pursued with a view to defining protein-RNA interactions in viroid replication in chloroplasts.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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