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Comparative analysis of the defence proteome of the model plant Arabidopsis thaliana

ReferenceP14635
Principal Investigator / Supervisor Professor Murray Grant
Co-Investigators /
Co-Supervisors
Professor John Mansfield
Institution Imperial College London
DepartmentAgricultural Sciences
Funding typeResearch
Value (£) 264,000
StatusCompleted
TypeResearch Grant
Start date 18/06/2001
End date 17/08/2004
Duration38 months

Abstract

In this project we will document changes in the Arabidopsis leaf proteome after challenge by avirulent, virulent and hrp mutant strains of Pseudomonas syringae pv. tomato (DC3000). Expression proteomics, utilising 2-D gel electrophoresis and mass spectroscopy following sub-cellular fractionation, will be used to establish a global picture of how components of the defence proteome change during susceptible or resistant interactions (both gene-for-gene and innate immunity). Our comparative studies will facilitate an understanding of signalling hierarchies activated or suppressed during the plants perception of an invading microbe, and allow determination of post-translational modifications not evident from traditional transcriptome approaches.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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