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Functional and genomic analysis of the disulphide stress response of Streptomyces coelicolor A3(2)
Reference
P14618
Principal Investigator / Supervisor
Dr Mark Paget
Co-Investigators /
Co-Supervisors
Institution
University of Sussex
Department
Biology and Environmental Science
Funding type
Research
Value (£)
166,064
Status
Completed
Type
Research Grant
Start date
16/07/2001
End date
16/11/2004
Duration
40 months
Abstract
The sigma factor SigR modulates the thioredoxin system in response to unwanted intracellular disulphide bond formation (disulphide stress) in Streptomyces coelicolor. We have identified an additional 29 SigR- dependent genes, most with unknown function. We propose to 1) use DNA microarrays to study the global response to disulphide stress, 2) characterise selected SigR-dependent genes by mutational and phenotypic analyses, 3) apply a random screen to identify new genes involved in controlling the thiol-disulphide redox balance, and 4) determine the extent of disulphide stress during growth and stationary phase. It is anticipated that these approaches will provide important insights into how actinomycetes control their thiol- disulphide redox status.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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