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Identification of foreign replicons and host genes naturally targeted by post-transcriptional gene silencing

Reference	P14588
Principal Investigator / Supervisor	Professor Sir David Baulcombe
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	173,580
Status	Completed
Type	Research Grant
Start date	01/05/2001
End date	01/05/2004
Duration	36 months

Abstract

Post-transcriptional gene silencing (PTGS) has emerged as an important cellular defence mechanism. Exclusively associated with PTGS is the accumulation of a novel class of short RNA molecules, derived from the targeted sequence, but always of the same size (approx. 25nt.) We propose three complementary approaches to identify the natural targets of PTGS using 25nt RNA as unique molecular markers of PTGS: 1) to test presence of 25nt RNA by Northern blotting using a range of candidate sequences (including viruses and transposons); 2) to identify less obvious targets of PTGS by labelling the 25nt RNA fraction and using this to probe libraries of plant sequences and 3) by directly cloning the 25nt fraction. These experiments will establish which parasitic elements are targeted by PTGS and which host signalling pathways exploit PTGS.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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