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Elucidating the pathway for S lignin biosynthesis in vivo using self-processing polyproteins

Reference	P14559
Principal Investigator / Supervisor	Professor Claire Halpin
Co-Investigators / Co-Supervisors	Professor Martin Ryan
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	93,482
Status	Completed
Type	Research Grant
Start date	01/12/2000
End date	01/09/2002
Duration	21 months

Abstract

Biochemical evidence suggests that the current model for syringyl biosynthesis is incorrect or incomplete. We have transgenic plants and constructs that are ideal for investigating the syringyl lignin pathway in vivo. Transgenic plants with deficiencies in CAD or CCR enzymes, deposit modified lignins. Unusual 'signature' phenolics are detected in these lignins by the sophisticated technique, thioacidolysis. Thus sinapaldehyde is a signature for severe CAD deficiency and ferulate/sinapate are signatures for severe CCR deficiency. Overexpressing F5H, the rate limiting enzyme in syringyl lignin biosynthesis, in these CAD or CCR deficient plants, will alter the proportions of the signature species in different ways depending on the true position at which F5H acts on the lignin pathway. By monitoring these changes we will be able to elucidate the pathway(s) to syringyl lignin used in vivo.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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