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Regulatory phosphorylation of phosphoenolpyruvate carboxykinase in plants

Reference	P14423
Principal Investigator / Supervisor	Professor Richard Leegood
Co-Investigators / Co-Supervisors	Professor Julie Gray, Dr Robert Walker
Institution	University of Sheffield
Department	Animal and Plant Sciences
Funding type	Research
Value (£)	192,276
Status	Completed
Type	Research Grant
Start date	01/05/2001
End date	01/05/2004
Duration	36 months

Abstract

The aim of the work is to investigate the regulatory properties of phosphoenolpyruvate carboxykinase (PCK) in plants and to investigate the factors regulating the phosphorylation of PCK in leaves of the C4 plant, Panicum maximum. We will (i) investigate whether or not PCK is phosphorylated at more than one site and identify the phosphorylation site(s) (ii) evaluate what signals regulate phosphorylation/dephosphorylation using intact leaves and bundle sheath strands (which contain both the kinase and target enzyme) (iii) determine the effects of phosphorylation on the regulatory properties of PCK and (iv) use site-directed mutagenesis to manipulate the regulatory properties of PCK.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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