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Functional analysis of transposon-tagged gametophytic fertility genes in Arabidopsis

Reference	P13877
Principal Investigator / Supervisor	Professor David Twell
Co-Investigators / Co-Supervisors	Dr Eric Lalanne
Institution	University of Leicester
Department	Biology
Funding type	Research
Value (£)	221,064
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	01/11/2003
Duration	36 months

Abstract

The proposed experiments will improve the understanding of molecular mechanisms underlying fertilisation processes in plants, and are intended to exploit the results of an on-going pump-prime programme focused on the isolation of mutants of the programic phase in Arabidopsis. Our key objective is to characterise the molecular and cellular basis of expression and function of three male-specific transposon-tagged gametophytic programic phase genes, PGP1, PGP2, and PGP3. pgp mutants fail in aspects of male post-pollination events from pollen germination to gamete recognition. Our specific aims are: 1) to confirm the identity and function of the disrupted genes; 2) to determine the expression profile and cellular localisation of PGP proteins (immunolocalisation and GFP localisation); and 3) to investigate in detail the action of PGP1 using genetic (ectopic expression) and biochemical approaches (co-immunoprecipitation and two-hybrid system).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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