Award details

Regulation and role of alanine synthesis in pea bacteroids

ReferenceP13752
Principal Investigator / Supervisor Professor Philip Poole
Co-Investigators /
Co-Supervisors
Institution University of Reading
DepartmentAnimal and Microbial Sciences
Funding typeResearch
Value (£) 265,920
StatusCompleted
TypeResearch Grant
Start date 06/11/2000
End date 06/11/2003
Duration36 months

Abstract

We have recently shown that symbiotic nitrogen fixation results in the export of both ammonia and alanine by Rhizobium bacteroids. The ratio between ammonia and alanine is likely to vary as a function of a number of variables. This overturns the long established principal that bacteroids only excrete ammonia and the plant carries out all nitrogen assimilation. Furthermore, we propose that alanine synthesis by bacteroids is part of a homeostatic mechanism to regulate both carbon and nitrogen metabolism in bacteroids. Here we propose to investigate how alanine synthesis and secretion is regulated and measure its impact on plant growth. In addition using 15N2 labelling, we propose to directly measure alanine export in planta.

Summary

unavailable
Committee Closed Committee - Plant & Microbial Sciences (PMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
terms and conditions of use (opens in new window)
export PDF file