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Motility temperature and quorum sensing in Yersinia pseudotuberculosis

Reference	P13751
Principal Investigator / Supervisor	Professor Paul Williams
Co-Investigators / Co-Supervisors	Dr Steven Atkinson, Professor Miguel Camara, Professor Renee Sockett
Institution	University of Nottingham
Department	Sch of Pharmacy
Funding type	Research
Value (£)	214,768
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

In Yersinia pseudotuberculosis, two N-acylhomoserine lactone (AHL)-dependent quorum sensing loci are involved in the temperature-dependent repression of motility and clumping. A third quorum sensing system based on luxS homologue has also been identified. We propose to build on these observations by determining how: (i) the quorum sensing circuitry is organised, (ii) flagellar motility is repressed and (iii) temperature controls which AHLs are synthesised. To address these questions we will: 1) Construct single and double mutants in each of the genes coding for LuxR (YpsR and YtbR), LuxI (YpsI and YtbI) and LuxS homologues and analyse their phenotypes; 2) Investigate the expression of flia and flhDC in single and double mutants; 3) Identify the target genes regulated by the quorum sensing loci; and 4) Establish the mechanism by which temperature controls the product output from the AHL synthases, YpsI and Ytb1.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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